Research

Did you know that Regenexx physicians have published over 44% of all orthopedic stem cell research? We are the only orthopedic stem cell providers with this volume of data to draw upon. As leaders in regenerative medicine, our physicians are dedicated to their patients, their research, and the advancement of Regenexx procedures.

Stem Cell

Platelet-Rich Plasma


A Case Series of Percutaneous Treatment of Non-Union Fractures with Autologous, Culture Expanded, Bone Marrow Derived, Mesenchymal Stem Cells and Platelet Lysate

Christopher J. Centeno, John R. Schultz.

Abstract

Background: Current treatment options for stable non-union fractures represent major clinical challenges, and are a major health issue. Fracture treatment can take many forms, usually requiring bone grafting and/or revisions of the fracture with open reduction and internal fixation (ORIF).

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Clinical Percutaneous Implantation of Autologous Culture-Expanded MSCs into Peripheral Joints

Christopher J. Centeno, John R. Schultz.

Abstract

Mesenchymal stem cells (MSCs) show great clinical potential as therapeutic agents in regenerative medicine. These adult stem cells can be easily isolated from many areas in the body, and many animal studies have demonstrated their multipotency in terms of differentiating into muscle, bone, cartilage, tendon, and various cells of internal organs.

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Intra-discal injection of autologous, hypoxic cultured bone marrow-derived mesenchymal stem cells in five patients with chronic lower back pain: a long-term safety and feasibility study.

Christopher J. Centeno, John R. Schultz.

Abstract

Background: Chronic low back pain due to disc degeneration represents a major social and economic burden worldwide. The current standard of care is limited to symptomatic relief and no current approved therapy promotes disc regeneration. Bone marrow-derived mesenchymal stem cells (MSCs) are easily accessible and well characterized.

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Percutaneous injection of autologous, culture-expanded mesenchymal stem cells into carpometacarpal hand joints: a case series with an untreated comparison group.

Christopher J. Centeno.

Abstract

In the present study, we describe six patients who received autologous mesenchymal stem cell (MSC) therapy for symptomatic carpometacarpal (CMC) joint and hand osteoarthritis (OA). Six patients who received injections of adult autologous culture expanded MSCs in their thumb CMC joints were followed for 1 year posttreatment, and matched with four procedure candidates who remained untreated.

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Safety and complications reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique.

Christopher J. Centeno, John R. Schultz.

Abstract

Methods: Between 2006 and 2010, two groups of patients were treated for various orthopedic conditions with culture-expanded, autologous, bone marrow-derived MSCs (group 1: n=50; group 2: n=290-one patient in both groups). Cells were cultured in monolayer culture flasks using an autologous platelet lysate technique and re-injected into peripheral joints or into intervertebral discs with use of c-arm fluoroscopy.

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Safety of intra-articular cell-therapy with culture-expanded stem cells in humans: a systematic literature review.

References to Centeno.

Abstract

Background: An important goal of stem cell research in orthopaedics is to develop clinically relevant techniques that could be applied to heal cartilage or joint pathology. Stem cell treatment in orthopaedics for joint pathology is promising since these cells have the ability to modulate different processes in the various tissues of the joint simultaneously.

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Treatment of lumbar degenerative disc disease-associated radicular pain with culture-expanded autologous mesenchymal stem cells: a pilot study on safety and efficacy.

Christopher J. Centeno, Matthew Hyzy, Jason Markle, Christopher Williams.

Abstract

Background: Degenerative disc disease (DDD) is a common cause of lower back pain with radicular symptoms and has a significant socioeconomic impact given the associated disability. Limited effective conservative therapeutic options result in many turning to surgical alternatives for management, which vary in the rate of success and also carry an increased risk of morbidity and mortality associated with the procedures.

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A Dose Response Analysis of a specific bone marrow concentrate treatment protocol for Knee OA

Christopher J. Centeno, Jamil Bashir.

Abstract

Background: Prior studies describing the treatment of symptomatic knee osteoarthritis with injections of bone marrow concentrate have provided encouraging results. The relationship between the cellular dose contained within the bone marrow concentrate and efficacy of the treatment, however, is unclear.

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A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions.

Christopher J. Centeno.

Abstract

Introduction: The purpose of the present investigation is to report on detailed complications among a much larger group of 2372 orthopaedic patients treated with stem cell injections who were followed in a treatment registry for up to nine years.

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A Prospective Multi-site Registry Study of a Specific Protocol of Autologous Bone Marrow Concentrate for the Treatment of Shoulder Rotator Cuff Tears and Osteoarthritis.

Christopher J. Centeno, Matthew Hyzy, Jamil Bashir.

Abstract

Introduction: Shoulder pain is a common musculoskeletal complaint in the general population. Bone marrow concentrate (BMC) injections offer promising potential as a minimally invasive approach for treatment of shoulder pain in degenerative disease.

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Amide-Type Local Anesthetics and Human Mesenchymal Stem Cells: Clinical Implications for Stem Cell Therapy

Christopher J. Centeno

Abstract

In the realm of regenerative medicine, human mesenchymal stem cells (hMSCs) are gaining attention as a cell source for the repair and regeneration of tissues spanning an array of medical disciplines. In orthopedics, hMSCs are often delivered in a site-specific manner at the area of interest and may require the concurrent application of local anesthetics (LAs).

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Anterior Cruciate Ligament (ACL) Tears treated with Percutaneous Injection of Autologous Bone Marrow Nucleated Cells: A Case Series.

Christopher J. Centeno, John Pitts.

Abstract

Introduction: This was a prospective case series designed to investigate treatment for anterior cruciate ligament (ACL) tears using an injection of autologous bone marrow concentrate.

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Clinical challenges and opportunities of mesenchymal stem cells in musculoskeletal medicine.

Christopher J. Centeno.

Abstract

The use of stem cells in orthopedics has been researched for many years, with robust animal data that show efficacy in cartilage healing, tendon repair, and intervertebral disk treatment. Early clinical data are also just starting to be published, and these results are encouraging.

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Correction to: A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions.

Christopher J. Centeno.

Abstract

The published online version contains mistake in Acknowledgment Section. The author name "Steve Gorin, M.D." should have been "Steven Gorin, D.O, M.S.Ed.".

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Efficacy and Safety of Bone Marrow Concentrate for Osteoarthritis of the Hip; Treatment Registry Results for 196 Patients.

John Pitts.

Abstract

We investigated the efficacy and safety of autologous bone marrow concentrate (BMC) for the treatment of symptomatic hip osteoarthritis.

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Efficacy of Autologous Bone Marrow Concentrate for Knee Osteoarthritis with and without Adipose Graft.

Christopher J. Centeno, John Pitts.

Abstract

We investigated the use of autologous bone marrow concentrate (BMC) with and without an adipose graft, for treatment of knee osteoarthritis (OA). Methods. Treatment registry data for patients who underwent BMC procedures with and without an adipose graft were analyzed.

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Increased Knee Cartilage Volume in DJD using percutaneously implanted autologous MSC

Christopher J. Centeno, Jamil Bashir.

Abstract

Background: The ability to repair tissue via percutaneous means may allow interventional pain physicians to manage a wide variety of diseases including peripheral joint injuries and osteoarthritis. This review will highlight the developments in cellular medicine that may soon permit interventional pain management physicians to treat a much wider variety of clinical conditions and highlight an interventional case study using these technologies.

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Interventional Orthopedics in Pain Medicine Practice

Christopher J. Centeno, Matthew Hyzy, Christopher Williams

Abstract

Interventional pain physicians are in a unique place to take advantage of regenerative medicine technology to improve patient outcomes and decrease the invasiveness of orthopedic procedural care. However, that sea of change would take significant changes to the educational system similar to those established when interventional spine was first introduced as a subspecialty.

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Osteoblastic differentiation of human and equine adult bone marrow-derived mesenchymal stem cells when BMP-2 or BMP-7 homodimer genetic modification is compared to BMP-2/7 heterodimer genetic modification in the presence and absence of dexamethasone.

Christopher J. Centeno.

Abstract

Bone marrow-derived mesenchymal stem cells (BMDMSCs) have been targeted for use in enhancement of bone healing; and their osteogenic potential may be further augmented by genes encoding bone morphogenetic proteins (BMP's). The purpose of this study was to compare the effect of genetic modification of human and equine BMDMSCs with BMP-2 or -7 or BMP-2 and -7 on their osteoblastogenic differentiation in the presence or absence of dexamethasone.

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Partial Regeneration of the Humam Hip via Autologous Bone Marrow Nucleated Cell Transfer: Case Study

Christopher J. Centeno, John R. Schultz.

Abstract

The use of progenitor cells in regenerative medicine holds great promise for degenerative joint disease (1). Specifically, the mesenchymal stem cell (MSC) lineage has been used in animal models to regenerate articular cartilage and bone (2-15).

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Performing a Better Bone Marrow Aspiration.

Christopher J. Centeno, Mayo F. Friedlis.

Abstract

Bone marrow aspiration (BMA) is increasingly being used to harvest stem cells for use in regenerative medicine. The focus of BMA in interventional orthopedics is to maximize the yield of mesenchymal stem cells.

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Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration

Christopher J. Centeno.

Abstract

The number of Mesenchymal Stem/Stromal Cells (MSCs) in the human bone marrow (BM) is small compared to other cell types. BM aspirate concentration (BMAC) may be used to increase numbers of MSCs, but the composition of MSC subpopulations and growth factors after processing are unknown.

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Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells.

Christopher J. Centeno.

Abstract

Mesenchymal stem cells are pluripotent cells found in multiple human tissues including bone marrow, synovial tissues, and adipose tissues. They have been shown to differentiate into bone, cartilage, muscle, and adipose tissue and represent a possible promising new therapy in regenerative medicine.

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Safety and regulatory issues regarding stem cell therapies: one clinic's perspective.

Christopher J. Centeno.

Abstract

Stem cells therapies have been in preclinical development for the past 2 decades. A rapidly evolving regulatory landscape has restrained many of these technologies from advancing from the bench to the bedside.

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Symptomatic anterior cruciate ligament tears treated with percutaneous injection of autologous bone marrow concentrate and platelet products: a non-controlled registry study.

Christopher J. Centeno, Matthew Hyzy, Jason Markle, Christopher Williams.

Abstract

Background: Bone marrow concentrate (BMC) has shown promise in the treatment of several orthopedic conditions. This registry study investigated the use of autologous BMC and platelet products for percutaneous anterior cruciate ligament (ACL) treatment.

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The Use of Mesenchymal Stem Cells In Orthopedics - review of the literature, Current research, and Regulatory Landscape

Christopher J. Centeno.

Abstract

Mesenchymal stem cells (MSCs) show promising clinical potential as multi-potent therapeutic agents in regenerative medicine, including a number of orthopedic applications. A comprehensive review of the medical literature regarding the pre-clinical and early clinical use of MSCs demonstrates that they are likely to be effective cellular repair agents for cartilage and joint injuries.

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A Randomized Controlled Trial of the Treatment of Rotator Cuff Tears with Bone Marrow Concentrate and Platelet Products Compared to Exercise Therapy: A Midterm Analysis

Christopher J. Centeno.

Abstract

Injectable regenerative therapies such as bone marrow concentrate (BMC) and platelet-rich plasma (PRP) may represent a safe alternative in the treatment of rotator cuff tears. This is a midterm review of a randomized, crossover trial comparing autologous BMC and platelet product injections versus exercise therapy in the treatment of partial and full-thickness supraspinatus tears.

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A specific protocol of autologous bone marrow concentrate and platelet products versus exercise therapy for symptomatic knee osteoarthritis: a randomized controlled trial with 2 year follow-up

Christopher J. Centeno, John R. Schultz.

Abstract

Background: Cell-based therapies have shown promise for the treatment of knee osteoarthritis (OA). The current study compared exercise therapy to autologous bone marrow concentrate (BMC) and platelet products for knee OA treatment.

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Fluoroscopically guided cervical prolotherapy for instability with blinded pre and post radiographic reading.

Christopher J. Centeno.

Abstract

Several authors have postulated that cervical instability is a major cause of traumatic spinal pain.

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Sclerotherapy of Baker's cyst with imaging confirmation of resolution.

Christopher J. Centeno, John R. Schultz.

Abstract

Baker's cysts are commonly encountered in pain management practices..

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Can the Regenexx-PL procedure replace steroid epidurals?

Christopher J. Centeno.

Abstract

The Regenexx-PL procedure removes the concentrated healing growth factors from platelets and makes them immediately available to cells.

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Platelet lysates from aged donors promote human tenocyte proliferation and migration in a concentrationdependent manner

Christopher J. Centeno.

Abstract

Objectives: Platelet-rich plasma (PRP) is being used increasingly often in the clinical setting to treat tendon-related pathologies. Yet the optimal PRP preparations to promote tendon healing in different patient populations are poorly defined.

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PL disc outcomes

Christopher J. Centeno.

Abstract

PL stands for “Platelet Lysate”. We take our novel Super Concentrated Platelet (SCP) mix and break open all of the platelets to get the growth factors out. Why? Just like an immediate release pill, sometimes it’s better to have more growth factors on the ground to help stimulate cells toward repair.

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The use of lumbar epidural injection of platelet lysate for treatment of radicular pain.

Christopher J. Centeno, Matthew Hyzy, Jason Markle, Christopher Williams

Abstract

Background: Epidural steroid injections (ESI) are the most common pain management procedure performed in the US, however evidence of efficacy is limited. In addition, there is early evidence that the high dose of corticosteroids used can have systemic side effects.

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